ARG44672
anti-HERC5 antibody
anti-HERC5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes HERC5 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2a |
| 靶点名称 | HERC5 |
| 抗原物种 | Human |
| 偶联标记 | Un-conjugated |
| 別名 | HECT domain and RCC1-like domain-containing protein 5; EC 6.3.2.-; CEB1; E3 ISG15--protein ligase HERC5; CEBP1; Cyclin-E-binding protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A purification |
| 缓冲液 | PBS with 0.09% sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HERC5 |
| 全名 | HECT and RLD domain containing E3 ubiquitin protein ligase 5 |
| 背景介绍 | This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008] |
| 生物功能 | Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15. [UniProt] |
| 细胞定位 | Cytoplasm, perinuclear region. Note=Associated with the polyribosomes, probably via the 60S subunit. [UniProt] |
| 预测分子量 | 117 kDa |
| 翻译后修饰 | N-glycosylation enhances cell surface expression and lengthens receptor half-life by preventing degradation in the ER. |
检测图片 (3) Click the Picture to Zoom In
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ARG44672 anti-HERC5 antibody IHC-P image
Immunohistochemistry: Human Testis stained with ARG44672 anti-HERC5 antibody at 5 µg/mL dilution.
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ARG44672 anti-HERC5 antibody WB image
Western blot: HEK293 stained with ARG44672 anti-HERC5 antibody at 1 µg/mL dilution.
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ARG44672 anti-HERC5 antibody IP image
Immunoprecipitation: HEK293 lysate immunoprecipitated with 2.5 µg of ARG44672 anti-HERC5 antibody.
