ARG54212
anti-HLA G antibody [87G]
anti-HLA G antibody [87G] for CyTOF®-candidate,ELISA,Flow cytometry,Functional study,IHC-Frozen sections and Human
Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [87G] recognizes HLA G |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Ms, Rat |
| 应用 | CyTOF®-candidate, ELISA, FACS, FuncSt, IHC-Fr |
| 特异性 | The clone 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. 87G blocks interaction of HLA-G with inhibitory receptors. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 87G |
| 同位型 | IgG2a |
| 靶点名称 | HLA G |
| 抗原物种 | Human |
| 抗原 | HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin. |
| 偶联标记 | Un-conjugated |
| 別名 | HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G |
应用说明
| 应用建议 |
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| 应用说明 | IHC-Fr: Incubated for 20 min at 25°C. ELISA: The clone 87G has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody W6/32. Functional studies: The clone 87G blocks interaction of HLA-G with inhibitory receptors. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | FACS and ELISA: JEG-3 human choriocarcinoma epithelial cells IHC-Fr: extravillous cytotrophoblast |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P17693 Human HLA class I histocompatibility antigen, alpha chain G |
|---|---|
| 基因名称 | HLA-G |
| 全名 | major histocompatibility complex, class I, G |
| 背景介绍 | HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt] |
| 产品亮点 | Related products: HLA G antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| 研究领域 | Immune System antibody |
| 预测分子量 | 38 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG54212 anti-HLA G antibody [87G] Functional study image
Functional study: Analysis of cytolytical activity of Human polyclonal NK cells on target melanoma cells (M8 cell line) transfected with empty vector (Lane 1) or HLA-G1 cDNA (Lane 2-6). Blocking antibodies: Lane (1,2): none Lane (3): Isotype Mouse IgG2a control Lane (4): ARG54212 anti-HLA G antibody [87G] (5): F(ab´)2 fragment of ARG54212.
