ARG10093
anti-HLA G antibody [MEM-G/9] (Azide free)
anti-HLA G antibody [MEM-G/9] (Azide free) for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human
Immune System antibody
概述
| 产品描述 | Azide free Mouse Monoclonal antibody [MEM-G/9] recognizes HLA G |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Ms |
| 应用 | ELISA, FACS, ICC/IF, IHC-Fr, IP |
| 特异性 | The clone MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003). |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-G/9 |
| 同位型 | IgG1 |
| 靶点名称 | HLA G |
| 抗原物种 | Human |
| 抗原 | Recombinant human HLA-G refolded with beta2-microglobulin and peptide. |
| 偶联标记 | Un-conjugated |
| 別名 | HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G |
应用说明
| 应用建议 |
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| 应用说明 | ELISA: Application note: The clone MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with clone B2M-01 or with clone W6/32. Coating antibody (10 μg/ml); Detection antibody (biotin or peroxidase conjugate; 1 μg/ml) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | FACS: JEG-3 Human choriocarcinoma cell line |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | 0.2 µm filter sterilized. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P17693 Human HLA class I histocompatibility antigen, alpha chain G |
|---|---|
| 基因名称 | HLA-G |
| 全名 | major histocompatibility complex, class I, G |
| 背景介绍 | HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt] |
| 研究领域 | Immune System antibody |
| 预测分子量 | 38 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG10093 anti-HLA G antibody [MEM-G/9] (Azide free) ICC/IF image
Immunofluorescence: HLA-G1 transfectants stained with ARG10093 anti-HLA G antibody [MEM-G/9] (Azide free) (green).
