ARG66339
anti-HRP antibody [SQab1887]
anti-HRP antibody [SQab1887] for Flow cytometry,ICC/IF,Western blot and Other
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1887] recognizes HRP |
|---|---|
| 反应物种 | Other |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1887 |
| 同位型 | IgG |
| 靶点名称 | HRP |
| 抗原物种 | Others |
| 抗原 | Full length native protein (purified) corresponding to HRP. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 预测分子量 | 39 kDa |
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检测图片 (3) Click the Picture to Zoom In
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ARG66339 anti-HRP antibody [SQab1887] ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66339 anti-HRP antibody [SQab1887] (green) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66339 anti-HRP antibody [SQab1887] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66339 anti-HRP antibody [SQab1887] (red) at 1:50 dilution in 1x PBS/1% BSA for 60 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66339 anti-HRP antibody [SQab1887] WB image
Western blot: 20 ng of HRP protein stained with ARG66339 anti-HRP antibody [SQab1887] at 1:10000 dilution.
