ARG10169
anti-Helicobacter pylori CagA antibody [10E9]
anti-Helicobacter pylori CagA antibody [10E9] for ELISA,Western blot and Helicobacter pylori
Microbiology and Infectious Disease antibody
概述
| 产品描述 | Mouse Monoclonal antibody [10E9] recognizes Helicobacter pylori CagA |
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| 反应物种 | H. pylori |
| 应用 | ELISA, WB |
| 特异性 | Cross-reaction with other proteins has not been identified. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 10E9 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | Helicobacter pylori CagA |
| 抗原 | A highly immunogenic 32kD CagA fragment, recombinant |
| 偶联标记 | Un-conjugated |
应用说明
| 应用说明 | ELISA: Clone 10E9 can be used as capture antibody in sandwich ELISA. Anti-CagA clone 10E9 coated wells detect recombinant CagA antigen in combination with HRP conjugated anti-CagA clone 5C6, clone 3C10 and clone 3C1. In addition, clone 10E9 selectively detected cell lysate of a CagA containing H. pylori strain when HRP conjugated anti-CagA clone 3C10 was used. Western Blot: This clone detects the 32 kD band corresponding to the molecular weight of the recombinant CagA antigen (Immunogen). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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属性
| 形式 | Liquid |
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| 纯化 | Protein G affinity purified |
| 缓冲液 | 0.01M PBS (pH 7.0) |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains. |
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| 产品亮点 | Related products: Helicobacter pylori antibodies; Helicobacter pylori ELISA Kits; Helicobacter pylori Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Tools for studying H. pylori diseases |
| 研究领域 | Microbiology and Infectious Disease antibody |
