ARG65382
anti-Helios antibody [22F6]
anti-Helios antibody [22F6] for CyTOF®-candidate,Flow cytometry and Human,Mouse
Controls and Markers antibody; Gene Regulation antibody; Immune System antibody
概述
| 产品描述 | Hamster Monoclonal antibody [22F6] recognizes Helios |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | CyTOF®-candidate, FACS |
| 特异性 | The armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor expressed in some hematopoietic stem cells, and at high levels in thymicderived regulatory T cells. |
| 宿主 | Hamster |
| 克隆 | Monoclonal |
| 克隆号 | 22F6 |
| 同位型 | IgG |
| 靶点名称 | Helios |
| 抗原 | Peptide coresponding to the amino acids 51-107 of Helios |
| 偶联标记 | Un-conjugated |
| 別名 | HELIOS; ZNF1A2; Ikaros family zinc finger protein 2; ANF1A2; Zinc finger protein Helios; ZNFN1A2 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Staining method: - Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. at RT in the dark; 100 µl of peripheral blood. - Add 3 ml of PBS with 1% BSA, centrifugate at 300g and discard the supernatant. Further steps perform on ice and with ice-cold reagents. - Resuspend the cells in 5 ml of cold fixation solution (Miltenyi Biotec) and incubate for 30 min. on ice. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 5 ml of ice-cold PBS with 1% BSA. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 5 ml of ice-cold permeabilization solution (Miltenyi Biotec) and incubate 5 min. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 80µl of ice-cold permeabilization solution and add 20 µl of FcR blocking solution and incubate for 5 min. (4°C, in the dark). - Perform intracellular staining of Helios for 30 min. (4°C, in the dark) with 10 µl of anti-Helios PE antibody (22F6) in 100 µl. - Add 2 ml of ice-cold permeabilization solution and incubate for 5 min. at 4°C in the dark. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 3 ml of ice-cold PBS with 1% BSA. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 150 µl of ice-cold PBS with 1% BSA and measure on a FACS device with appropriate setting as soon as possible. Keep cold until measuring. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IKZF2 |
| 全名 | IKAROS family zinc finger 2 (Helios) |
| 背景介绍 | Helios, also known as IKZF2 (Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells. |
| 生物功能 | Associates with Ikaros at centromeric heterochromatin. [UniProt] |
| 产品亮点 | Related products: Helios antibodies; Anti-Hamster IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| 研究领域 | Controls and Markers antibody; Gene Regulation antibody; Immune System antibody |
| 预测分子量 | 60 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG65382 anti-Helios antibody [22F6] FACS image
Flow Cytometry: Human peripheral stained with ARG65382 anti-Helios antibody [22F6] at 8 μg/ml dilution.
