ARG55677
anti-Hemoglobin beta antibody
anti-Hemoglobin beta antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Hemoglobin beta |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Bov, Chk, Pig, Rb |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Hemoglobin beta |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 80-107 (C-terminus) of Human Hemoglobin beta. |
| 偶联标记 | Un-conjugated |
| 別名 | CD113t-C; beta-globin; Beta-globin; Hemoglobin beta chain; Hemoglobin subunit beta |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Human heart |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HBB |
| 全名 | hemoglobin, beta |
| 背景介绍 | The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in oxygen transport from the lung to the various peripheral tissues. LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure. Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation. [UniProt] |
| 预测分子量 | 16 kDa |
| 翻译后修饰 | Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus. S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2). Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. |
检测图片 (3) Click the Picture to Zoom In
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ARG55677 anti-Hemoglobin beta antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver tissue stained with ARG55677 anti-Hemoglobin beta antibody .
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ARG55677 anti-Hemoglobin beta antibody WB image
Western blot: 20 µg of Human heart lysate stained with ARG55677 anti-Hemoglobin beta antibody at 1:2000 dilution.
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ARG55677 anti-Hemoglobin beta antibody FACS image
Flow Cytometry: K562 cells stained with ARG55677 anti-Hemoglobin beta antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC labelled secondary antibody.
