ARG43029
anti-Histone H4 acetyl (Lys12) antibody
anti-Histone H4 acetyl (Lys12) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Histone H4 acetyl (Lys12) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Histone H4 |
| 抗原物种 | Human |
| 抗原 | Synthetic acetylated peptide around Lys12 of Human Histone H4 (NP_001029249.1). |
| 偶联标记 | Un-conjugated |
| 別名 | H4/I; H4C1; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FI; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; HIST1H4B |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | C2C12 + TSA |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | H4C2 |
|---|---|
| 全名 | H4 clustered histone 2 |
| 背景介绍 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| 生物功能 | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [UniProt] |
| 细胞定位 | Nucleus. Chromosome. [UniProt] |
| 预测分子量 | 11 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG43029 anti-Histone H4 acetyl (Lys12) antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells were treated by TSA (1 µM) at 37°C for 18 hours. Cells were stained with ARG43029 anti-Histone H4 acetyl (Lys12) antibody at 1:100 dilution.
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ARG43029 anti-Histone H4 acetyl (Lys12) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spinal cord tissue stained with ARG43029 anti-Histone H4 acetyl (Lys12) antibody at 1:200 dilution.
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ARG43029 anti-Histone H4 acetyl (Lys12) antibody WB image
Western blot: C2C12 cells were untreated (-) or treated (+) by TSA (1 µM) at 37°C for 18 hours. 25 µg of cell lysates stained with ARG43029 anti-Histone H4 acetyl (Lys12) antibody at 1:1000 dilution.
