ARG43026
anti-Histone H4 acetyl (Lys16) antibody
anti-Histone H4 acetyl (Lys16) antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Histone H4 acetyl (Lys16) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Histone H4 |
| 抗原物种 | Human |
| 抗原 | Synthetic acetylated peptide around Lys16 of Human Histone H4 (NP_001029249.1). |
| 偶联标记 | Un-conjugated |
| 別名 | H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; HIST2H4; HIST2H4A |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | C2C12 + TSA |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | H4C14 |
| 全名 | H4 clustered histone 14 |
| 背景介绍 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Aug 2015] |
| 生物功能 | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [UniProt] |
| 细胞定位 | Nucleus. Chromosome. [UniProt] |
| 预测分子量 | 11 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG43026 anti-Histone H4 acetyl (Lys16) antibody ICC/IF image
Immunofluorescence: U2OS cells were treated by TSA (1 µM) at 37°C for 18 hours. Cells were stained with ARG43026 anti-Histone H4 acetyl (Lys16) antibody at 1:100 dilution.
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ARG43026 anti-Histone H4 acetyl (Lys16) antibody WB image
Western blot: C2C12 cells were untreated (-) or treated (+) by TSA. 25 µg of cell lysates stained with ARG43026 anti-Histone H4 acetyl (Lys16) antibody at 1:1000 dilution.
