ARG54173
anti-Hsp 60 antibody
anti-Hsp 60 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody
参考文献1
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Hsp 60 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | Hsp 60 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human Hsp60 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | Heat shock protein 60; HuCHA60; 60 kDa heat shock protein, mitochondrial; GROEL; P60 lymphocyte protein; 60 kDa chaperonin; HSP-60; Mitochondrial matrix protein P1; HLD4; SPG13; HSP60; Chaperonin 60; Hsp60; CPN60; HSP65 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 2.6 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSPD1 |
| 全名 | heat shock 60kDa protein 1 (chaperonin) |
| 背景介绍 | Implicated in mitochondrial protein import and macromolecular assembly.May facilitate the correct folding of imported proteins.May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. |
| 生物功能 | Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt] |
| 细胞定位 | Mitochondrion matrix. |
| 产品亮点 | Related Antibody Duos and Panels: ARG30271 Mitochondrial Marker Antibody Panel (Cytochrome C, COX4, HSP60) Related products: Hsp60 antibodies; Hsp60 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody |
| 预测分子量 | 61 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG54173 anti-Hsp 60 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54173 anti-Hsp 60 antibody at 1:100 dilution. Left: primary antibody (green). Middle: DAPI (blue). Right: Merge.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG54173 anti-Hsp 60 antibody WB image
Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG54173 anti-Hsp 60 antibody at 1:1000 dilution.
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ARG54173 anti-Hsp 60 antibody WB image
Western blot: 15 µg of H1299, U87, HeLa, Jurkat and Raw264.7 cell lysates stained with ARG54173 anti-Hsp 60 antibody.
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ARG54173 anti-Hsp 60 antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54173 anti-Hsp 60 antibody.
客户反馈
Excellent
anti-Hsp 60 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
anti-Hsp 60 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
anti-Hsp 60 antibody
Application：WB
Sample：293T, Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-Hsp 60 antibody
Application：WB
Sample：A: Mouse brain, B: Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
RSV replication is promoted by autophagy-mediated inhibition of apoptosis.
WB / Human
