ARG65733
anti-Hsp 60 antibody
anti-Hsp 60 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Hsp 60 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Hsp 60 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around aa. 480-560 (C-terminus) of Human Hsp60. |
| 偶联标记 | Un-conjugated |
| 別名 | Heat shock protein 60; HuCHA60; 60 kDa heat shock protein, mitochondrial; GROEL; P60 lymphocyte protein; 60 kDa chaperonin; HSP-60; Mitochondrial matrix protein P1; HLD4; SPG13; HSP60; Chaperonin 60; Hsp60; CPN60; HSP65 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 0.5% BSA and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA, 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSPD1 |
| 全名 | heat shock 60kDa protein 1 (chaperonin) |
| 背景介绍 | This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010] |
| 生物功能 | Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt] |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody |
| 预测分子量 | 61 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG65733 anti-Hsp 60 antibody WB image
Western blot: LOVO, NIH-3T3, and Rat muscle cell lysates (left to right) stained with ARG65733 anti-Hsp 60 antibody.
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ARG65733 anti-Hsp 60 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung carcinoma tissue stained wih ARG65733 anti-Hsp 60 antibody at 1:50 dilution.
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ARG65733 anti-Hsp 60 antibody ICC/IF image
Immunocytochemistry: HepG2 cells stained with ARG65733 anti-Hsp 60 antibody at 1:100 dilution.
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ARG65733 anti-Hsp 60 antibody WB image
Western blot: COLO205 cells stained with ARG65733 anti-Hsp 60 antibody.
客户反馈
Excellent
anti-Hsp 60 antibody
Application：WB
Sample：U87-MG
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
