ARG55956

Name: anti-Hsp 60 antibody [LK1]
Brand: arigo Biolaboratories
SKU: ARG55956
Price: 389 USD
Availability: InStock
Rating: 4.8 (4 reviews)

anti-Hsp 60 antibody [LK1]

Datasheet

订购信息

包装

价格 (USD)

50 μg

389

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [LK1] recognizes Hsp 60
反应物种	Hu, Ms, Rat
不反应物种	Bacteria
应用	FACS, ICC/IF, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	LK1
同位型	IgG1, kappa
靶点名称	Hsp 60
抗原物种	Human
抗原	Recombinant Human Hsp60 protein.
偶联标记	Un-conjugated
別名	Heat shock protein 60; HuCHA60; 60 kDa heat shock protein, mitochondrial; GROEL; P60 lymphocyte protein; 60 kDa chaperonin; HSP-60; Mitochondrial matrix protein P1; HLD4; SPG13; HSP60; Chaperonin 60; Hsp60; CPN60; HSP65

应用说明

应用建议

应用	推荐稀释比
FACS	1 - 2 µg/10^6 cells
ICC/IF	2 - 5 µg/ml
IHC-P	2 - 5 µg/ml
WB	1 - 2 µg/ml

应用说明

IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
抗菌剂	0.05% Sodium azide
稳定剂	0.1 mg/ml BSA
浓度	0.2 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 15510 Mouse HSPD1 GeneID: 3329 Human HSPD1 GeneID: 63868 Rat HSPD1 Swiss-port # P10809 Human 60 kDa heat shock protein, mitochondrial Swiss-port # P63038 Mouse 60 kDa heat shock protein, mitochondrial Swiss-port # P63039 Rat 60 kDa heat shock protein, mitochondrial More
基因名称	HSPD1
全名	heat shock 60kDa protein 1 (chaperonin)
背景介绍	This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
生物功能	Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]
细胞定位	Mitochondria in cytoplasm
研究领域	Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody
预测分子量	61 kDa

检测图片 (4) Click the Picture to Zoom In

ARG55956 anti-Hsp 60 antibody [LK1] WB image

Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG55956 anti-Hsp 60 antibody [LK1] at 0.4 µg/ml dilution.
ARG55956 anti-Hsp 60 antibody [LK1] IHC-P image

Immunohistochemistry: Formalin-fixed, paraffin-embedded Human breast carcinoma stained with ARG55956 anti-Hsp 60 antibody [LK1].
ARG55956 anti-Hsp 60 antibody [LK1] ICC/IF image

Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55956 anti-Hsp 60 antibody [LK1] at 1:200 dilution. Left: primary antibody (green). Middle: DAPI (blue). Right: Merge.

Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
ARG55956 anti-Hsp 60 antibody [LK1] IHC-P image

Immunohistochemistry: Formalin-fixed, paraffin-embedded pancreas tissue stained with ARG55956 anti-Hsp 60 antibody [LK1].