ARG55413
anti-IFIH1 / MDA5 antibody
anti-IFIH1 / MDA5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody; Immune System antibody; Microbiology and Infectious Disease antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes IFIH1 / MDA5 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IFIH1 / MDA5 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (16 aa) within the last 50 aa of Human MDA5. |
| 偶联标记 | Un-conjugated |
| 別名 | Interferon-induced helicase C domain-containing protein 1; Murabutide down-regulated protein; SGMRT1; EC 3.6.4.13; RIG-I-like receptor 2; MDA5; Clinically amyopathic dermatomyositis autoantigen 140 kDa; AGS7; MDA-5; CADM-140 autoantigen; Melanoma differentiation-associated protein 5; IDDM19; Interferon-induced with helicase C domain protein 1; RNA helicase-DEAD box protein 116; Helicard; RLR-2; Hlcd; Helicase with 2 CARD domains |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Daudi Cell Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8R5F7 Mouse Interferon-induced helicase C domain-containing protein 1 Swiss-port # Q9BYX4 Human Interferon-induced helicase C domain-containing protein 1 |
|---|---|
| 基因名称 | IFIH1 |
| 全名 | interferon induced with helicase C domain 1 |
| 背景介绍 | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012] |
| 生物功能 | Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines. [UniProt] |
| 产品亮点 | Related products: IFIH1 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | Gene Regulation antibody; Immune System antibody; Microbiology and Infectious Disease antibody |
| 预测分子量 | 117 kDa |
| 翻译后修饰 | Sumoylated. Sumoylation positively regulates its role in type I interferon induction and is enhanced by PIAS2-beta. Ubiquitinated by RNF125, leading to its degradation by the proteasome (PubMed:17460044). USP17/UPS17L2-dependent deubiquitination positively regulates the receptor (PubMed:20368735). During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation. |
检测图片 (3) Click the Picture to Zoom In
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ARG55413 anti-IFIH1 / MDA5 antibody WB image
Western blot: Daudi cell lysate stained with ARG55413 anti-IFIH1 / MDA5 antibody at (A) 1, (B) 2 and (C) 4 µg/ml dilution.
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ARG55413 anti-IFIH1 / MDA5 antibody IHC image
Immunohistochemistry: Human lymph node tissue stained with ARG55413 anti-IFIH1 / MDA5 antibody at 5 µg/ml dilution.
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ARG55413 anti-IFIH1 / MDA5 antibody ICC/IF image
Immunofluorescence: Human Lymph Node cells stained with ARG55413 anti-IFIH1 / MDA5 antibody at 20 µg/ml dilution.
