ARG63323
anti-IFIH1 / MDA5 antibody
anti-IFIH1 / MDA5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Monkey
Gene Regulation antibody; Immune System antibody; Microbiology and Infectious Disease antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes IFIH1 / MDA5 |
|---|---|
| 反应物种 | Hu, Mk |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IFIH1 / MDA5 |
| 抗原物种 | Human |
| 抗原 | SNGYSTDENFRYL-C |
| 偶联标记 | Un-conjugated |
| 別名 | Interferon-induced helicase C domain-containing protein 1; Murabutide down-regulated protein; SGMRT1; EC 3.6.4.13; RIG-I-like receptor 2; MDA5; Clinically amyopathic dermatomyositis autoantigen 140 kDa; AGS7; MDA-5; CADM-140 autoantigen; Melanoma differentiation-associated protein 5; IDDM19; Interferon-induced with helicase C domain protein 1; RNA helicase-DEAD box protein 116; Helicard; RLR-2; Hlcd; Helicase with 2 CARD domains |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9BYX4 Human Interferon-induced helicase C domain-containing protein 1 |
|---|---|
| 背景介绍 | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012] |
| 产品亮点 | Related products: IFIH1 antibodies; Anti-Goat IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | Gene Regulation antibody; Immune System antibody; Microbiology and Infectious Disease antibody |
| 预测分子量 | 117 kDa |
| 翻译后修饰 | Sumoylated. Sumoylation positively regulates its role in type I interferon induction and is enhanced by PIAS2-beta. Ubiquitinated by RNF125, leading to its degradation by the proteasome (PubMed:17460044). USP17/UPS17L2-dependent deubiquitination positively regulates the receptor (PubMed:20368735). During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation. |
检测图片 (1) Click the Picture to Zoom In
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ARG63323 anti-IFIH1 / MDA5 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Pancreas. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63323 anti-IFIH1 / MDA5 antibody at 10 µg/ml dilution followed by HRP-staining.
