ARG65395
anti-IFN gamma antibody [4S.B3]
anti-IFN gamma antibody [4S.B3] for CyTOF®-candidate,ELISA,Flow cytometry,Functional study,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Radioimmunoassay,Western blot and Human,Primates
Cancer antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [4S.B3] recognizes IFN gamma |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | CyTOF®-candidate, ELISA, FACS, FuncSt, ICC/IF, IHC-P, IP, RIA, WB |
| 特异性 | The clone 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4S.B3 |
| 同位型 | IgG1 |
| 靶点名称 | IFN gamma |
| 抗原物种 | Human |
| 抗原 | Interferon gamma derived from human leukocytes |
| 偶联标记 | Un-conjugated |
| 別名 | IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI |
应用说明
| 应用建议 |
|
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | ELISA: Used as detection antibody in combination with capture antibody NIB42 Functional study: Neutralization (weak). FACS: Intracellular staining, permeabilization is needed. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||||||||||||
| 实际分子量 | ~16-18 kDa (monomer); 34-36 kDa (dimer) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The Interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells. |
| 生物功能 | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt] |
| 产品亮点 | Related products: IFN gamma antibodies; IFN gamma ELISA Kits; IFN gamma Duos / Panels; IFN gamma recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines CyTOF-candidate Antibodies |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. |
