ARG63880
anti-IFT88 / Polaris antibody
anti-IFT88 / Polaris antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes IFT88 / Polaris |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Dog, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2) |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IFT88 / Polaris |
| 抗原物种 | Human |
| 抗原 | C-KKRIDEDDFADEE |
| 偶联标记 | Un-conjugated |
| 別名 | Recessive polycystic kidney disease protein Tg737 homolog; hTg737; D13S1056E; Intraflagellar transport protein 88 homolog; TG737; TPR repeat protein 10; TTC10; Tetratricopeptide repeat protein 10; DAF19 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13099 Human Intraflagellar transport protein 88 homolog Swiss-port # Q61371 Mouse Intraflagellar transport protein 88 homolog |
|---|---|
| 背景介绍 | This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| 产品亮点 | Related products: Anti-Goat IgG secondary antibodies; Related news: Gene therapy for retinitis pigmentosa (RP) |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 94 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG63880 anti-IFT88 / Polaris antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63880 anti-IFT88 / Polaris antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63880 anti-IFT88 / Polaris antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63880 anti-IFT88 / Polaris antibody at 3 µg/ml dilution followed by HRP-staining.
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ARG63880 anti-IFT88 / Polaris antibody WB image
Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG63880 anti-IFT88 / Polaris antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
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ARG63880 anti-IFT88 / Polaris antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.5% Triton. Cells were stained with ARG63880 anti-IFT88 / Polaris antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG63880 anti-IFT88 / Polaris antibody WB image
Western blot: 35 µg of HepG2 (A) and NIH/3T3 (B) cell lysates (in RIPA buffer) stained with ARG63880 anti-IFT88 / Polaris antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
