ARG51651

anti-IKB alpha phospho (Ser32 / Ser36) antibody

Datasheet

参考文献3

订购信息

包装

价格 (USD)

100 μl

399

50 μl

279

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes IKB alpha phospho (Ser32 / Ser36)
反应物种	Hu, Ms, Rat
应用	ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	IKB alpha
抗原物种	Human
抗原	Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IκB-α.
偶联标记	Un-conjugated
別名	NFKBI; I-kappa-B-alpha; IkB-alpha; NF-kappa-B inhibitor alpha; Major histocompatibility complex enhancer-binding protein MAD3; IKBA; IkappaBalpha; MAD-3

应用说明

应用建议

应用	推荐稀释比
ICC/IF	1:100 - 1:200
IHC-P	1:50 - 1:100
WB	1:500 - 1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
缓冲液	PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 18035 Mouse NFKBIA GeneID: 25493 Rat NFKBIA GeneID: 4792 Human NFKBIA Swiss-port # P25963 Human NF-kappa-B inhibitor alpha Swiss-port # Q63746 Rat NF-kappa-B inhibitor alpha Swiss-port # Q9Z1E3 Mouse NF-kappa-B inhibitor alpha More
基因名称	NFKBIA
全名	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
背景介绍	Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
生物功能	Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. [UniProt]
产品亮点	Related Antibody Duos and Panels: ARG30037 Phospho IKB alpha Antibody Duo (Total, pS32/36) ARG30205 NFkB Activation Antibody Panel Related products: IKB alpha antibodies; IKB alpha Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Treatment of Obesity with Celastrol Tumor microenvironments are shown to affect progression of several cancer subtypes Immune signaling protein TLR4 has opposing roles in breast cancer development
研究领域	Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; IκB alpha degradation Study antibody; NF-kB Activation Study antibody
预测分子量	36 kDa
翻译后修饰	Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation. Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability. Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36. Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.

检测图片 (4) Click the Picture to Zoom In

ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody WB image

Western blot: Rat cardiac stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody at dilution.

From Yongjun Mao et al. Biomed Pharmacother. (2020), doi: 10.1016/j.biopha.2019.109280, Fig. 6D.
ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody WB image

Western blot: Extracts from HeLa cells untreated or treated with TNF stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody.
ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody (left) or the same antibody preincubated with blocking peptide (right).
ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody ICC/IF image

Immunofluorescence: methanol-fixed HeLa cells stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody.