ARG66320

Name: anti-IKK gamma antibody [SQab1868]
Brand: arigo Biolaboratories
SKU: ARG66320
Price: 399 USD
Availability: InStock
Rating: 5.0 (2 reviews)

anti-IKK gamma antibody [SQab1868]

Datasheet

订购信息

包装

价格 (USD)

100 μl

399
350

50 μl

279
246

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Recombinant Rabbit Monoclonal antibody [SQab1868] recognizes IKK gamma
反应物种	Hu, Ms, Rat
应用	FACS, IHC-P, IP, WB
宿主	Rabbit
克隆	Monoclonal
克隆号	SQab1868
同位型	IgG
靶点名称	IKK gamma
抗原物种	Human
抗原	Synthetic peptide around the N-terminus of IKK gamma.
偶联标记	Un-conjugated
別名	I-kappa-B kinase subunit gamma; IkB kinase-associated protein 1; Fip3p; NF-kappa-B essential modifier; IKK-gamma; IP; NF-kappa-B essential modulator; NEMO; ZC2HC9; AMCBX1; IP2; IKKG; IP1; FIP3; IMD33; IkB kinase subunit gamma; IKKAP1; IPD2; FIP-3; Inhibitor of nuclear factor kappa-B kinase subunit gamma

应用说明

应用建议

应用	推荐稀释比
FACS	1:200 - 1:1000
IHC-P	1:100 - 1:200
IP	1:50
WB	1:2000 - 1:5000

应用说明

IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

实际分子量

37-60 kDa

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂	0.01% Sodium azide
稳定剂	40% Glycerol and 0.05% BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 16151 Mouse IKBKG GeneID: 309295 Rat IKBKG GeneID: 8517 Human IKBKG Swiss-port # O88522 Mouse NF-kappa-B essential modulator Swiss-port # Q6TMG5 Rat NF-kappa-B essential modulator Swiss-port # Q9Y6K9 Human NF-kappa-B essential modulator More
基因名称	IKBKG
全名	inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
背景介绍	IKK gamma is a regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]
生物功能	IKK gamma: Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination. [UniProt]
预测分子量	48 kDa
翻译后修饰	Phosphorylation at Ser-68 attenuates aminoterminal homodimerization. Polyubiquitinated on Lys-285 through 'Lys-63'; the ubiquitination is mediated by NOD2 and RIPK2 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Polyubiquitinated on Lys-399 through 'Lys-63'; the ubiquitination is mediated by BCL10, MALT1 and TRAF6 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Monoubiquitinated on Lys-277 and Lys-309; promotes nuclear export. Polyubiquitinated through 'Lys-27' by TRIM23; involved in antiviral innate and inflammatory responses. Linear polyubiquitinated on Lys-111, Lys-143, Lys-226, Lys-246, Lys-264, Lys-277, Lys-285, Lys-292, Lys-302, Lys-309 and Lys-326; the head-to-tail polyubiquitination is mediated by the LUBAC complex and plays a key role in NF-kappa-B activation. Polyubiquitinated on Lys-309 and Lys-321 via 'Lys-27'-linked ubiquitin by Shigella flexneri E3 ubiquitin-protein ligase ipah9.8, leading to its degradation by the proteasome. Deubiquitinated by USP10 in a TANK-dependent and -independent manner, leading to the negative regulation of NF-kappaB signaling upon DNA damage (PubMed:25861989). Sumoylated on Lys-277 and Lys-309 with SUMO1; the modification results in phosphorylation of Ser-85 by ATM leading to a replacement of the sumoylation by mono-ubiquitination on these residues. Neddylated by TRIM40, resulting in stabilization of NFKBIA and down-regulation of NF-kappa-B activity. [UniProt]

检测图片 (5) Click the Picture to Zoom In

ARG66320 anti-IKK gamma antibody [SQab1868] FACS image

Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66320 anti-IKK gamma antibody [SQab1868] (red) at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
ARG66320 anti-IKK gamma antibody [SQab1868] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded liver tissue stained with ARG66320 anti-IKK gamma antibody [SQab1868] at 1:200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
ARG66320 anti-IKK gamma antibody [SQab1868] WB image

Western blot: 10 µg of Jurkat, K562, HeLa and NIH/3T3 cell lysates stained with ARG66320 anti-IKK gamma antibody [SQab1868] at 1:2000 dilution.
ARG66320 anti-IKK gamma antibody [SQab1868] IP image

Immunoprecipitation: 0.4 mg of HeLa whole cell lysate immunoprecipitated (1:50) and stained with ARG66320 anti-IKK gamma antibody [SQab1868]. 1) ARG66320 IP in HeLa whole cell, 2) PBS instead of ARG66320 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 10 µg (input).
ARG66320 anti-IKK gamma antibody [SQab1868] WB image

Western blot: 10 µg of PC-12, PC-3, HepG2 and Mouse kidney lysates stained with ARG66320 anti-IKK gamma antibody [SQab1868] at 1:2000 dilution.