ARG21420
anti-IL10 antibody [JES3-9D7] (low endotoxin)
anti-IL10 antibody [JES3-9D7] (low endotoxin) for ELISA,ELISPOT,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Rhesus Monkey,Virus
概述
| 产品描述 | Azide free and low endotoxin Rat Monoclonal antibody [JES3-9D7] recognizes IL10 |
|---|---|
| 反应物种 | Hu, R. Mk, Virus |
| 应用 | ELISA, ELISPOT, FACS, IHC-Fr, IHC-P, Neut, WB |
| 特异性 | IL10 antibody clone JES3-9D7 reacts to Human/Viral/Rhesus/Cynomolgus/Sooty Mangabey IL-10, the antibody also reacts to vIL-10 from Epstein-Barr virus (EBV) BCRF1 gene. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | JES3-9D7 |
| 同位型 | IgG1, kappa |
| 靶点名称 | IL10 |
| 抗原物种 | Human |
| 抗原 | COS-expressed human IL-10 |
| 偶联标记 | Un-conjugated |
| 別名 | IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10 |
应用说明
| 应用建议 |
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| 应用说明 | ELISA: The clone JES3-9D7 may be paired with the purified clone JES3-12G8 in a sandwich ELISA. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | Low endotoxin |
| 缓冲液 | PBS |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IL10 |
| 全名 | interleukin 10 |
| 背景介绍 | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011] |
| 生物功能 | Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt] |
| 预测分子量 | 21 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG21420 anti-IL10 antibody [JES3-9D7] (low endotoxin) standard curve image
ELISA: Human IL-10 detected by ARG21419 anti-IL10 antibody [JES3-9D7] as capture antibody ( ARG21420: Low endotoxin version), and ARG21422 anti-IL10 antibody [JES3-12G8] (Biotin) as detection antibody, follow by incubation with streptavidin-HRP.
