ARG22432
anti-IL12B / IL12 p40 antibody [CC301] (low endotoxin)
anti-IL12B / IL12 p40 antibody [CC301] (low endotoxin) for ELISA,ELISPOT,Flow cytometry,Functional study,Western blot and Human,Bovine,Sheep
概述
| 产品描述 | Azide free and low endotoxin Mouse Monoclonal antibody [CC301] recognizes IL12B / IL12 p40 This antibody recognizes the p40 subunit of bovine interleukin-12, and binds to both the free subunit and the intact heterodimer. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A.Mouse anti Bovine Interleukin-12/23 antibody, clone CC301 may be used as a capture antibody in an ELISA in combination with biotinylated Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 as a detection reagent. |
|---|---|
| 反应物种 | Hu, Bov, Sheep |
| 不反应物种 | Ms |
| 应用 | ELISA, ELISPOT, FACS, FuncSt, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | CC301 |
| 同位型 | IgG2a |
| 靶点名称 | IL12B / IL12 p40 |
| 抗原物种 | Bovine |
| 抗原 | Recombinant Bovine IL12B / IL12 p40. |
| 偶联标记 | Un-conjugated |
| 別名 | CLMF; Interleukin-12 subunit beta; NK cell stimulatory factor chain 2; NKSF; CLMF p40; NKSF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF2; IL-12 subunit p40; IMD29; IMD28; IL-12B |
应用说明
| 应用建议 |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | FACS: Membrane permeabilisation is required for this application. Arigo recommends the use of Leucoperm for this purpose. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | Low endotoxin |
| 缓冲液 | PBS |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IL12B |
| 全名 | interleukin 12B |
| 背景介绍 | This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. [UniProt] |
| 预测分子量 | 37 kDa |
| 翻译后修饰 | Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified. |
