ARG56805

anti-IL13 antibody (Biotin)

anti-IL13 antibody (Biotin) for ELISA,Western blot and Rat

概述

产品描述 Biotin-conjugated Rabbit Polyclonal antibody recognizes IL13
反应物种 Rat
应用 ELISA, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 IL13
抗原物种 Rat
抗原 E.coli derived Recombinant Rat IL-13 (113 aa).
(TPGPVRRSTS PPVALRELIE ELSNITQDQK TSLCNSSIVW SVDLTAGGFC AALESLTNIS SCNAIHRTQR ILNGLCNQKA SDVASSPPDT KIEVAQFISK LLNYSKQLFR YGH)
偶联标记 Biotin
別名 Interleukin-13; P600; IL-13

应用说明

应用建议
应用 推荐稀释比
ELISADirect: ~ 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56696 as a capture antibody
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified by affinity chromatography.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 116553 Rat IL13

Swiss-port # P42203 Rat Interleukin-13

基因名称 Il13
全名 interleukin 13
背景介绍 This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008]
生物功能 Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. [UniProt]
预测分子量 16 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG56805 anti-IL13 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Rat IL-13 stained with ARG56805 anti-IL13 antibody (Biotin), under reducing conditions.

  • ARG56805 anti-IL13 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Rat IL-13 stained with ARG56805 anti-IL13 antibody (Biotin), under non-reducing conditions.

  • ARG56805 anti-IL13 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56805 anti-IL13 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56696 anti-IL13 antibody as a capture antibody. Results of a typical standard run with optical density.