ARG56591

anti-IL6 antibody [1.2-2B11-2G10]

Datasheet

参考文献1

订购信息

包装

价格 (USD)

500 μg

495

250 μg

342

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [1.2-2B11-2G10] recognizes IL6
反应物种	Hu, Rat
应用	ELISA, ICC/IF, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	1.2-2B11-2G10
同位型	IgG1, kappa
靶点名称	IL6
抗原物种	Human
抗原	E.coli derived Recombinant Human IL-6. (PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM)
偶联标记	Un-conjugated
別名	B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2

应用说明

应用建议

应用	推荐稀释比
ELISA	1 - 2 µg/ml
ICC/IF	Assay-dependent
IHC-P	0.125 µg/ml
WB	0.20 - 0.40 µg/ml

应用说明

Sandwich ELISA (Capture antibody - Detection antibody):
ARG56591 (1 - 2 µg/ml) - ARG56736 (0.1 - 0.2 µg/ml)
ARG56591 (1 - 2 µg/ml) - ARG56627 (0.1 - 0.2 µg/ml)

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.2)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 24498 Rat IL6 GeneID: 3569 Human IL6 Swiss-port # P05231 Human Interleukin-6 Swiss-port # P20607 Rat Interleukin-6
基因名称	IL6
全名	interleukin 6
背景介绍	This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
生物功能	Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt]
产品亮点	Related products: IL6 antibodies; IL6 ELISA Kits; IL6 recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines
预测分子量	24 kDa
翻译后修饰	N- and O-glycosylated.

检测图片 (4) Click the Picture to Zoom In

ARG56591 anti-IL6 antibody [1.2-2B11-2G10] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56591 anti-IL6 antibody [1.2-2B11-2G10] concentration is 0.125 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
ARG56591 anti-IL6 antibody [1.2-2B11-2G10] WB image

Western blot: 250 - 0.24 ng (left to right) of Human IL-6 stained with ARG56591 anti-IL6 antibody [1.2-2B11-2G10], under reducing conditions.
ARG56591 anti-IL6 antibody [1.2-2B11-2G10] standard curve image

Sandwich ELISA: ARG56591 anti-IL6 antibody [1.2-2B11-2G10] as a capture antibody at 1 - 2 µg/ml dilution combined with ARG56736 anti-IL6 antibody (Biotin) as a detection antibody at 0.1 - 0.2 µg/ml dilution. Results of a typical standard run with optical density.
ARG56591 anti-IL6 antibody [1.2-2B11-2G10] WB image

Western blot: 250 - 0.24 ng (left to right) of Human IL-6 stained with ARG56591 anti-IL6 antibody [1.2-2B11-2G10], under non-reducing conditions.