ARG56625
anti-IL6 antibody
anti-IL6 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Mouse
参考文献3
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes IL6 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ELISA, IHC-P, Neut, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IL6 |
| 抗原物种 | Human |
| 抗原 | E.coli derived Recombinant Human IL-6. (PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM) |
| 偶联标记 | Un-conjugated |
| 別名 | B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 25 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IL6 |
| 全名 | interleukin 6 |
| 背景介绍 | This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011] |
| 生物功能 | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt] |
| 产品亮点 | Related products: IL6 antibodies; IL6 ELISA Kits; IL6 recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines |
| 预测分子量 | 24 kDa |
| 翻译后修饰 | N- and O-glycosylated. |
检测图片 (4) Click the Picture to Zoom In
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ARG56625 anti-IL6 antibody WB image
Western blot: 50 µg of A549 untreated or treated with Herba C. The blots were stained with ARG56625 anti-IL6 antibody at 1:2000 dilution.
Observed band MW: 25 kDa.
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ARG56625 anti-IL6 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56625 anti-IL6 antibody concentration is 1 µg/ml - 5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56625 anti-IL6 antibody standard curve image
Sandwich ELISA: ARG56625 anti-IL6 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56735 anti-IL6 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
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ARG56625 anti-IL6 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56625 anti-IL6 antibody concentration is 1 µg/ml - 5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
客户反馈
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anti-IL6 antibody
Application：WB
Sample：A549 untreated or treated with Herba C
Sample Loading Amount：50 µg
Primary Antibody Dilution Factor：1:2000
Primary Antibody Incubation Time：2 hours
Primary Antibody Incubation Temperature：25 ºC
文献引用
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