ARG40603
anti-IRE1a phospho (Ser724) antibody
anti-IRE1a phospho (Ser724) antibody for Western blot and Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes IRE1a phospho (Ser724) |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Hu, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IRE1a |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Ser724 of Human IRE1a. |
| 偶联标记 | Un-conjugated |
| 別名 | Ire1-alpha; Serine/threonine-protein kinase/endoribonuclease IRE1; IRE1a; Endoplasmic reticulum-to-nucleus signaling 1; EC 3.1.26.-; IRE1; Inositol-requiring protein 1; IRE1P; EC 2.7.11.1; hIRE1p |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | NIH/3T3 | ||||
| 实际分子量 | 130 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9EQY0 Mouse Serine/threonine-protein kinase/endoribonuclease IRE1 |
|---|---|
| 基因名称 | ERN1 |
| 全名 | endoplasmic reticulum to nucleus signaling 1 |
| 背景介绍 | The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008] |
| 生物功能 | Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. [UniProt] |
| 细胞定位 | Endoplasmic reticulum membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 110 kDa |
| 翻译后修饰 | Autophosphorylated. ADP-ribosylated by PARP16 upon ER stress, which increases both kinase and endonuclease activities. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG40603 anti-IRE1a phospho (Ser724) antibody WB image
Western blot: NIH/3T3 were stained with ARG40603 anti-IRE1a phospho (Ser724) antibody at 1:800 dilution.
