ARG57516

anti-IRF3 phospho (Ser396) antibody

anti-IRF3 phospho (Ser396) antibody for Western blot and Human,Pig

概述

产品描述 Rabbit Polyclonal antibody recognizes IRF3 phospho (Ser396)
反应物种 Hu, Pig
预测物种 Ms
应用 WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 IRF3
抗原物种 Human
抗原 Phospho-specific peptide around Ser396 of Human IRF3.
偶联标记 Un-conjugated
別名 IRF-3; Interferon regulatory factor 3

应用说明

应用建议
应用 推荐稀释比
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HCT116

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3661 Human IRF3

GeneID: 396656 Pig IRF3

Swiss-port # Q14653 Human Interferon regulatory factor 3

Swiss-port # Q764M6 Pig Interferon regulatory factor 3

基因名称 IRF3
全名 interferon regulatory factor 3
背景介绍 This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
生物功能 Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages. [UniProt]
产品亮点 Related products:
IRF3 antibodies; Anti-Rabbit IgG secondary antibodies;
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预测分子量 47 kDa
翻译后修饰 Constitutively phosphorylated on many Ser/Thr residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Phosphorylated at Ser-396 by IKBKE upon ssRNA viral infection. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. Phosphorylation at Ser-386 by TBK1 results in oligomerization. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection.

(Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.

Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.

ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG57516 anti-IRF3 phospho (Ser396) antibody WB image

    Western blot: 25 µg of HCT116 cell lysate stained with ARG57516 anti-IRF3 phospho (Ser396) antibody at 1:1000 dilution.