ARG11026
anti-ITK antibody
anti-ITK antibody for IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ITK |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ITK |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide taken within aa. 550-600 on Human ITK protein. |
| 偶联标记 | Un-conjugated |
| 別名 | LPFS1; Interleukin-2-inducible T-cell kinase; T-cell-specific kinase; Tyrosine-protein kinase ITK/TSK; PSCTK2; EMT; Kinase EMT; Tyrosine-protein kinase Lyk; LYK; IL-2-inducible T-cell kinase; EC 2.7.10.2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | Tris, HCl/glycine buffer (pH 7.4 - 7.8), cryo-protective agents, Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 30% Glycerol and 0.5% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITK |
| 全名 | IL2-inducible T-cell kinase |
| 背景介绍 | This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008] |
| 生物功能 | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. [UniProt] |
| 预测分子量 | 72 kDa |
| 翻译后修饰 | Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK. Subsequent autophosphorylation at Tyr-180 leads to the kinase activation. The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain. Ubiquitinated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG11026 anti-ITK antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse large intestine tissue stained with ARG11026 anti-ITK antibody at 1:100 dilution. (20X magnification)
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ARG11026 anti-ITK antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse large intestine tissue stained with ARG11026 anti-ITK antibody at 1:100 dilution. (40X magnification)
