ARG43799
anti-KMT4 / Dot1L antibody
anti-KMT4 / Dot1L antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes KMT4 / Dot1L |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KMT4 / Dot1L |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to Human KMT4 / Dot1L. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Histone-lysine N-methyltransferase, H3 lysine-79 specific |
| 別名 | KMT4; DOT1; Histone-lysine N-methyltransferase, H3 lysine-79 specific; H3-K79-HMTase; DOT1-like protein; Lysine N-methyltransferase 4; EC 2.1.1.43; Histone H3-K79 methyltransferase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa; Mouse testis | ||||||
| 实际分子量 | ~180 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 浓度 | Batch dependent |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8TEK3 Human Histone-lysine N-methyltransferase, H3 lysine-79 specific |
|---|---|
| 基因名称 | DOT1L |
| 全名 | DOT1-like histone H3K79 methyltransferase |
| 背景介绍 | The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011] |
| 生物功能 | Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 165 kDa |
| 翻译后修饰 | Phosphoprotein |
检测图片 (3) Click the Picture to Zoom In
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ARG43799 anti-KMT4 / Dot1L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse testis tissue stained with ARG43799 anti-KMT4 / Dot1L antibody.
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ARG43799 anti-KMT4 / Dot1L antibody WB image
Western blot: 25 µg of HeLa cells stained with ARG43799 anti-KMT4 / Dot1L antibody at 1:1000 dilution.
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ARG43799 anti-KMT4 / Dot1L antibody WB image
Western blot: 25 µg of Raw263.7 cell lysate stained with ARG43799 anti-KMT4 / Dot1L antibody at 1:1000 dilution.
