ARG65620
anti-Ki-67 antibody [Ki-67]
anti-Ki-67 antibody [Ki-67] for CyTOF®-candidate,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Bovine
Microvascular Density Study antibody; Proliferation Marker antibody
概述
| 产品描述 | Mouse Monoclonal antibody [Ki-67] recognizes Ki-67 |
|---|---|
| 反应物种 | Hu, Bov |
| 应用 | CyTOF®-candidate, FACS, ICC/IF, IHC-P, WB |
| 特异性 | This antibody recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | Ki-67 |
| 同位型 | IgG1 |
| 靶点名称 | Ki-67 |
| 抗原物种 | Human |
| 抗原 | Nuclei of the Hodgkin lymphoma cell line L428 |
| 偶联标记 | Un-conjugated |
| 別名 | Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105 |
应用说明
| 应用建议 |
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| 应用说明 | Immunocytochemistry: paraformaldehyde fixation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MKI67 |
| 全名 | marker of proliferation Ki-67 |
| 背景介绍 | Ki-67 is a nuclear protein. It is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009] |
| 生物功能 | Ki-67 required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). [UniProt] |
| 产品亮点 | Related products: Ki67 antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| 研究领域 | Microvascular Density Study antibody; Proliferation Marker antibody |
| 预测分子量 | 359 kDa |
| 翻译后修饰 | Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. |
检测图片 (2) Click the Picture to Zoom In
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ARG65620 anti-Ki-67 antibody [Ki-67] ICC/IF image
Immunocytochemistry: U2OS cell line (human osteosarcoma) stained with ARG65620 anti-Ki-67 antibody [Ki-67] (green). Cell nuclei stained with DAPI (blue).
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ARG65620 anti-Ki-67 antibody [Ki-67] FACS image
Flow Cytometry: Human PHA stimulated peripheral whole blood staining of ARG65620 anti-Ki-67 antibody [Ki-67] at 0.6 μg/ml dilution.
