ARG66982
anti-Ki-67 antibody [SP6]
anti-Ki-67 antibody [SP6] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Microvascular Density Study antibody; Proliferation Marker antibody
概述
| 产品描述 | Rabbit Monoclonal antibody [SP6] recognizes Ki-67 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SP6 |
| 同位型 | IgG |
| 靶点名称 | Ki-67 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from C-terminus of human Ki-67 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300, 1%BSA and 20% Glycerol. |
| 抗菌剂 | 0.025% ProClin 300 |
| 稳定剂 | 1%BSA and 20% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MKI67 |
| 全名 | marker of proliferation Ki-67 |
| 背景介绍 | Ki-67 is a nuclear protein, which is expressed in the proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. |
| 细胞定位 | Nucleus |
| 产品亮点 | Related products: Ki67 antibodies; Ki67 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Understanding Your Cells: Choose the right markers SM5-1, a promising immunotherapy for Hepatocellular Carcinoma (HCC) Neuronal Development Marker Choose the Best ZIKA Virus Antibodies Fight microcephaly with arigo Tools for studying Exosomes |
| 研究领域 | Microvascular Density Study antibody; Proliferation Marker antibody |
| 预测分子量 | 345 kDa and 395 kDa |
| 翻译后修饰 | Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. |
检测图片 (3) Click the Picture to Zoom In
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ARG66982 anti-Ki-67 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG66982 anti-Ki-67 antibody at 1:100 dilution.
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ARG66982 anti-Ki-67 antibody WB image
Western blot: CT26 cell lysate stained with ARG66982 anti-Ki-67 antibody at 1:500 dilution, overnight at 4°C.
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ARG66982 anti-Ki-67 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse heart tissue stained with ARG66982 anti-Ki-67 antibody at 1:100 dilution.
