ARG51300

anti-LC3A antibody

anti-LC3A antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
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publication_link 参考文献1

概述

产品描述 Rabbit Polyclonal antibody recognizes LC3A
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 LC3A
抗原物种 Human
抗原 Peptide sequence around aa.5~10 (R-P-F-K-Q) derived from Human LC3A.
偶联标记 Un-conjugated
別名 MAP1A/MAP1B light chain 3 A; MAP1BLC3; LC3; MAP1 light chain 3-like protein 1; MAP1A/MAP1B LC3 A; Autophagy-related protein LC3 A; Microtubule-associated protein 1 light chain 3 alpha; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3A; LC3A; ATG8E; Autophagy-related ubiquitin-like modifier LC3 A

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:100 - 1:200
IHC-PAssay-dependent
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
缓冲液 PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 362245 Rat MAP1LC3A

GeneID: 66734 Mouse MAP1LC3A

GeneID: 84557 Human MAP1LC3A

基因名称 MAP1LC3A
全名 microtubule-associated protein 1 light chain 3 alpha
背景介绍 Probably involved in formation of autophagosomal vacuoles (autophagosomes).
生物功能 Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30273 LC3A and LC3B Antibody Duo
Related products:
LC3A antibodies; LC3A Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
预测分子量 14 kDa
翻译后修饰 The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.

检测图片 (4) Click the Picture to Zoom In

  • ARG51300 anti-LC3A antibody WB image

    Western blot: 30 µg of HeLa untreated or treated with CQ (50 µM) and stained with ARG51300 anti-LC3A antibody at 1:1000 dilution.

  • ARG51300 anti-LC3A antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with anti-LC3A antibody ARG51300.

  • ARG51300 anti-LC3A antibody WB image

    Western blot: 30 µg of HeLa cell lysate stained with ARG51300 anti-LC3A antibody at 1:500 dilution.

  • ARG51300 anti-LC3A antibody WB image

    Western blot: 30 µg of HeLa cells untreated or treated with Chloroquine at 50 µM or 100 µM (18 hr). The blots stained with ARG51300 anti-LC3A antibody at 1:500 dilution.

客户反馈

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anti-LC3A antibody

Application:WB

Sample:HeLa or HeLa CQ 50 µM

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-LC3A antibody

Application:WB

Sample:HeLa untreated or treated with Chloroquine at 50 uM, 100 uM (18hr)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-LC3A antibody

Application:WB

Sample:Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-LC3A antibody

Application:WB

Sample:Mouse brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Role of Autophagy in Acquired Cholesteatoma.

WB, IHC-P / Human

Li Quancheng et al.
Otol Neurotol.,  (2019)

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