ARG54669
anti-LC3C antibody
anti-LC3C antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes LC3C |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, ICC/IF, IHC-P, WB |
| 特异性 | MAP1LC3C antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3C are known to exist. MAP1LC3C antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3B. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LC3C |
| 抗原 | Synthetic peptide (18 aa) within the last 50 aa of Human MAP1LC3C. |
| 偶联标记 | Un-conjugated |
| 別名 | Microtubule-associated proteins 1A/1B light chain 3C; MAP1A/MAP1B light chain 3 C; ATG8J; Microtubule-associated protein 1 light chain 3 gamma; MAP1 light chain 3-like protein 3; MAP1A/MAP1B LC3 C; Autophagy-related protein LC3 C; LC3C; Autophagy-related ubiquitin-like modifier LC3 C |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Human Brain Tissue Lysate | ||||||||||
| 实际分子量 | 16 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9BXW4 Human Microtubule-associated proteins 1A/1B light chain 3C |
|---|---|
| 基因名称 | MAP1LC3C |
| 全名 | microtubule-associated protein 1 light chain 3 gamma |
| 背景介绍 | Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3C belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2). MAP1LC3C is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3C, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3). | |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 17 kDa |
| 翻译后修饰 | The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094). The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. |
检测图片 (3) Click the Picture to Zoom In
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ARG54669 anti-MAP1LC3C antibody ICC/IF image
Immunofluorescence: human brain tissue stained with ARG54669 anti-MAP1LC3C antibody at 20 μg/ml.
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ARG54669 anti-MAP1LC3C antibody IHC image
Immunohistochemistry: human brain tissue stained with ARG54669 anti-MAP1LC3C antibody at 5 μg/ml.
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ARG54669 anti-MAP1LC3C antibody WB image
Western blot: human brain tissue lysate stained with ARG54669 anti-MAP1LC3C antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
