ARG57101
anti-LDHA / Lactate Dehydrogenase antibody [1A4]
anti-LDHA / Lactate Dehydrogenase antibody [1A4] for Flow cytometry,ICC/IF,Western blot and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody
概述
| 产品描述 | Mouse Monoclonal antibody [1A4] recognizes LDHA / Lactate Dehydrogenase |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1A4 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | LDHA / Lactate Dehydrogenase |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-332 of Human LDHA |
| 偶联标记 | Un-conjugated |
| 別名 | PIG19; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59; HEL-S-133P; L-lactate dehydrogenase A chain; GSD11; LDHM; EC 1.1.1.27; LDH-M; LDH-A |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LDHA |
| 全名 | lactate dehydrogenase A |
| 背景介绍 | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008] |
| 研究领域 | Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody |
| 预测分子量 | 37 kDa |
| 翻译后修饰 | ISGylated. |
检测图片 (4) Click the Picture to Zoom In
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ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] at 1:500 (Red).
Hoechst 3342 (Blue) for nucleus staining.
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ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] FACS image
Flow Cytometry: HeLa cell line stained with ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
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ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] WB image
Western blot: 40 µg of 1) Jurkat, 2) 293T, and 3) A431 cell lysates stained with ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] at 1:1000.
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ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] WB image
Western blot: 35 µg of HeLa cell lysate stained with ARG57101 anti-LDHA / Lactate Dehydrogenase antibody [1A4] at 1:8000.
