ARG40322

anti-LDHA / Lactate Dehydrogenase antibody

anti-LDHA / Lactate Dehydrogenase antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody

概述

产品描述 Rabbit Polyclonal antibody recognizes LDHA / Lactate Dehydrogenase
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 LDHA / Lactate Dehydrogenase
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 1-332 of Human LDHA (NP_005557.1).
偶联标记 Un-conjugated
別名 PIG19; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59; HEL-S-133P; L-lactate dehydrogenase A chain; GSD11; LDHM; EC 1.1.1.27; LDH-M; LDH-A

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 NIH/3T3 and Jurkat
实际分子量 36 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 16828 Mouse LDHA

GeneID: 3939 Human LDHA

Swiss-port # P00338 Human L-lactate dehydrogenase A chain

Swiss-port # P06151 Mouse L-lactate dehydrogenase A chain

基因名称 LDHA
全名 lactate dehydrogenase A
背景介绍 The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]
细胞定位 Cytoplasm. [UniProt]
研究领域 Cancer antibody; Metabolism antibody; Signaling Transduction antibody; Lactate dehydrogenase antibody; Warburg Effect signaling pathway antibody
预测分子量 37 kDa
翻译后修饰 ISGylated. [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG40322 anti-LDHA / Lactate Dehydrogenase antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG40322 anti-LDHA / Lactate Dehydrogenase antibody.

  • ARG40322 anti-LDHA / Lactate Dehydrogenase antibody WB image

    Western blot: 25 µg of NIH/3T3 and Jurkat cell lysates stained with ARG40322 anti-LDHA / Lactate Dehydrogenase antibody at 1:1000 dilution.