ARG64029
anti-LEF1 antibody
anti-LEF1 antibody for Western blot and Human
Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes LEF1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Dog |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LEF1 |
| 抗原物种 | Human |
| 抗原 | C-QHEQRKEQEPKRPH |
| 偶联标记 | Un-conjugated |
| 別名 | Lymphoid enhancer-binding factor 1; TCF1-alpha; TCF7L3; T cell-specific transcription factor 1-alpha; LEF-1; TCF1ALPHA; TCF10 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9UJU2 Human Lymphoid enhancer-binding factor 1 |
|---|---|
| 背景介绍 | This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 44 kDa |
| 翻译后修饰 | Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway. |
检测图片 (2) Click the Picture to Zoom In
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ARG64029 anti-LEF1 antibody WB image
Western Blot: Daudi cell lysate (35 µg protein in RIPA buffer) stained with ARG64029 anti-LEF1 antibody at 1 µg/ml dilution.
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ARG64029 anti-LEF1 antibody WB image
Western blot: 35 µg of MOLT-4 cell lysate (in RIPA buffer) stained with ARG64029 anti-LEF1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
