ARG63966
anti-Leptin Receptor antibody
anti-Leptin Receptor antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Leptin Receptor |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Dog |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Leptin Receptor |
| 抗原物种 | Human |
| 抗原 | C-TQDDIEKHQSDAG |
| 偶联标记 | Un-conjugated |
| 別名 | LEPRD; CD antigen CD295; CD295; Leptin receptor; LEP-R; HuB219; OB-R; OBR; OB receptor |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010] |
| 产品亮点 | Related news: Studying obesity and appetite control by quantifying orexigenic and anorexigenic hormones; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 132 kDa |
| 翻译后修饰 | On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation,for interaction with SOCS3 and SOCS3 mediated inhibition of leptin signaling. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. Phosphorylation of Tyr-1079 has a more accessory role. |
检测图片 (4) Click the Picture to Zoom In
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ARG63966 anti-Leptin Receptor antibody WB image
Western Blot: Human Brain lysate (35 µg protein in RIPA buffer) stained with ARG63966 anti-Leptin Receptor antibody at 1 µg/ml dilution.
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ARG63966 anti-Leptin Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63966 anti-Leptin Receptor antibody at 4 µg/ml dilution followed by AP-staining.
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ARG63966 anti-Leptin Receptor antibody WB image
Western blot: 35 µg of Mouse brain (A) and Rat brain (B) lysates (in RIPA buffer) stained with ARG63966 anti-Leptin Receptor antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63966 anti-Leptin Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63966 anti-Leptin Receptor antibody at 4 µg/ml dilution followed by AP-staining.
