ARG56700
anti-Leptin antibody
anti-Leptin antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Leptin |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ELISA, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Leptin |
| 抗原物种 | Human |
| 抗原 | E.coli derived Recombinant Human Leptin. (MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC) |
| 偶联标记 | Un-conjugated |
| 別名 | Leptin; Obese protein; OB; LEPD; Obesity factor; OBS |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~15 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LEP |
| 全名 | leptin |
| 背景介绍 | This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008] |
| 生物功能 | May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. [UniProt] |
| 产品亮点 | Related news: Studying obesity and appetite control by quantifying orexigenic and anorexigenic hormones; |
| 预测分子量 | 19 kDa |
检测图片 (7) Click the Picture to Zoom In
-
ARG56700 anti-Leptin antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56700 anti-Leptin antibody concentration is 0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
-
ARG56700 anti-Leptin antibody WB image
Western blot: 250 - 0.24 ng of Human Leptin stained with ARG56700 anti-Leptin antibody, under reducing conditions.
-
ARG56700 anti-Leptin antibody WB image
Western blot: 250 - 0.24 ng of Human Leptin stained with ARG56700 anti-Leptin antibody, under reducing conditions.
-
ARG56700 anti-Leptin antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56700 anti-Leptin antibody concentration is 0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
-
ARG56700 anti-Leptin antibody WB image
Western blot: 250 - 0.24 ng of Human Leptin stained with ARG56700 anti-Leptin antibody, under non-reducing conditions.
-
ARG56700 anti-Leptin antibody WB image
Western blot: 250 - 0.24 ng of Human Leptin stained with ARG56700 anti-Leptin antibody, under non-reducing conditions.
-
ARG56700 anti-Leptin antibody standard curve image
Sandwich ELISA: ARG56700 anti-Leptin antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56810 anti-Leptin antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
