ARG56810

anti-Leptin antibody (Biotin)

anti-Leptin antibody (Biotin) for ELISA,Western blot and Human

概述

产品描述 Biotin-conjugated Rabbit Polyclonal antibody recognizes Leptin
反应物种 Hu
应用 ELISA, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Leptin
抗原物种 Human
抗原 E.coli derived Recombinant Human Leptin.
(MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC)
偶联标记 Biotin
別名 Leptin; Obese protein; OB; LEPD; Obesity factor; OBS

应用说明

应用建议
应用 推荐稀释比
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56700 as a capture antibody
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified by affinity chromatography.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3952 Human LEP

Swiss-port # P41159 Human Leptin

基因名称 LEP
全名 leptin
背景介绍 This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]
生物功能 May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. [UniProt]
预测分子量 19 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG56810 anti-Leptin antibody (Biotin) standard curve image

    Direct ELISA: ARG56810 anti-Leptin antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56810 anti-Leptin antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56810 anti-Leptin antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56700 anti-Leptin antibody as a capture antibody. Results of a typical standard run with optical density.