ARG63758
anti-Lipocalin 2 antibody
anti-Lipocalin 2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Lipocalin 2 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Lipocalin 2 |
| 抗原物种 | Human |
| 抗原 | C-AILREDKDPQKMY |
| 偶联标记 | Un-conjugated |
| 別名 | Siderocalin LCN2; Oncogene 24p3; MSFI; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; p25; Neutrophil gelatinase-associated lipocalin; 24p3; NGAL |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P80188 Human Neutrophil gelatinase-associated lipocalin |
|---|---|
| 基因名称 | LCN2 |
| 全名 | lipocalin 2 |
| 生物功能 | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 23 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG63758 anti-Lipocalin 2 antibody WB image
Western blot: Human Kidney lysate (35 µg protein in RIPA buffer) stained with ARG63758 anti-Lipocalin 2 antibody at 0.03 µg/ml dilution.
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ARG63758 anti-Lipocalin 2 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Spleen. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63758 anti-Lipocalin 2 antibody at 2.5 µg/ml dilution followed by AP-staining.
