ARG56964

anti-Liver FABP antibody [2G4]

Datasheet

参考文献1

订购信息

包装

价格 (USD)

50 μl

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [2G4] recognizes Liver FABP
反应物种	Hu
应用	FACS, ICC/IF, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	2G4
同位型	IgG1, kappa
靶点名称	Liver FABP
抗原物种	Human
抗原	Recombinant fragment around aa. 1-127 of Human Liver FABP.
偶联标记	Un-conjugated
別名	FABPL; Fatty acid-binding protein, liver; Liver-type fatty acid-binding protein; L-FABP; Fatty acid-binding protein 1

应用说明

应用建议

应用	推荐稀释比
FACS	Assay-dependent
ICC/IF	1:100
IHC-P	1:100
WB	1:1000 - 1:2000

应用说明

IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 2168 Human FABP1 Swiss-port # P07148 Human Fatty acid-binding protein, liver
基因名称	FABP1
全名	fatty acid binding protein 1, liver
背景介绍	This gene encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Mar 2011]
生物功能	Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. [UniProt]
预测分子量	14 kDa

检测图片 (4) Click the Picture to Zoom In

ARG56964 anti-Liver FABP antibody [2G4] ICC/IF image

Immunoflorescense: Hep3B cell line stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:100 (Green).

DAPI (Blue) for nucleus staining.
ARG56964 anti-Liver FABP antibody [2G4] FACS image

Flow Cytometry: Hep3B cell line stained with ARG56964 anti-Liver FABP antibody [2G4] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
ARG56964 anti-Liver FABP antibody [2G4] IHC-P image

Immunohistochemistry: Paraffin embedded sections of Human colon cancer tissue stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:100 for 2 hours at RT. Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
ARG56964 anti-Liver FABP antibody [2G4] WB image

Western blot: 1) 50 ng of FABP1 Recombinant protein, 2) 40 µg of HepG2 cell lysate, 3) 40 µg of Liver tissues lysate stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:1000.