ARG54031
anti-MAP2 antibody (N-term)
anti-MAP2 antibody (N-term) for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes MAP2 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | MAP2 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human MAP2 (N-terminus) protein fragments expressed in E.coli |
| 偶联标记 | Un-conjugated |
| 別名 | MAP2A; Microtubule-associated protein 2; MAP2C; MAP2B; MAP-2 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 300 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150mM NaCl, 0.2%Sodium azide, 0.1%BSA and 50%Glycerol |
| 抗菌剂 | 0.2%Sodium azide |
| 稳定剂 | 0.1%BSA, 50%Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MAP2 |
| 全名 | microtubule-associated protein 2 |
| 背景介绍 | The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization.They also seem to have a stiffening effect on microtubules. |
| 生物功能 | The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton |
| 产品亮点 | Related products: MAP2 antibodies; MAP2 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Neuronal Development Marker Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| 研究领域 | Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody |
| 预测分子量 | 200 kDa |
| 翻译后修饰 | Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity). |
检测图片 (3) Click the Picture to Zoom In
-
ARG54031 anti-MAP2 antibody (N-term) ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG54031 anti-MAP2 antibody (N-term) at 1:100 dilution.
-
ARG54031 anti-MAP2 antibody (N-term) WB image
Western blot: Mouse brain lysate stained with ARG54031 anti-MAP2 antibody (N-term) at 1:1000 dilution.
-
ARG54031 anti-MAP2 antibody (N-term) IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54031 anti-MAP2 antibody (N-term).
客户反馈
Good
anti-MAP2 antibody (N-term)
Application：WB
Sample：Mouse brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
