概述

产品描述	Rabbit Polyclonal antibody recognizes MAPK7 / ERK5
反应物种	Hu, Ms, Rat
应用	FACS, ICC/IF, IP, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	MAPK7 / ERK5
抗原物种	Human
抗原	Synthetic peptide of Human MAPK7 / ERK5.
偶联标记	Un-conjugated
別名	Extracellular signal-regulated kinase 5; EC 2.7.11.24; ERK4; ERK5; Big MAP kinase 1; BMK1; PRKM7; BMK-1; MAP kinase 7; MAPK 7; ERK-5; Mitogen-activated protein kinase 7

应用说明

应用建议	应用 推荐稀释比 FACS 1:50 ICC/IF 1:50 - 1:200 IP 1:50 WB 1:1000 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	HeLa
实际分子量	~ 110 kDa

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 23939 Mouse MAPK7 GeneID: 5598 Human MAPK7 Swiss-port # Q13164 Human Mitogen-activated protein kinase 7 Swiss-port # Q9WVS8 Mouse Mitogen-activated protein kinase 7
基因名称	MAPK7
全名	mitogen-activated protein kinase 7
背景介绍	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
生物功能	Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction. [UniProt]
细胞定位	Cytoplasm. Nucleus. Nucleus, PML body. Note=Translocates to the nucleus upon activation. [UniProt]
预测分子量	88 kDa
翻译后修饰	Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent. [UniProt]

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