ARG55687

anti-MDH1 antibody

anti-MDH1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes MDH1
反应物种 Hu
预测物种 Ms, Rat, Bov, Chk, Pig
应用 FACS, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 MDH1
抗原物种 Human
抗原 KLH-conjugated synthetic peptide corresponding to aa. 286-314 (C-terminus) of Human MDH1.
偶联标记 Un-conjugated
別名 Diiodophenylpyruvate reductase; MDHA; MOR2; HEL-S-32; Cytosolic malate dehydrogenase; EC 1.1.1.37; Malate dehydrogenase, cytoplasmic; EC 1.1.1.96; MDH-s; MGC:1375

应用说明

应用建议
应用 推荐稀释比
FACS1:10 - 1:50
IHC-P1:10 - 1:50
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HL-60

属性

形式 Liquid
纯化 This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
缓冲液 PBS and 0.09% (W/V) Sodium azide.
抗菌剂 0.09% (W/V) Sodium azide.
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 4190 Human MDH1

Swiss-port # P40925 Human Malate dehydrogenase, cytoplasmic

基因名称 MDH1
全名 malate dehydrogenase 1, NAD (soluble)
背景介绍 Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]
细胞定位 Cytoplasm.
产品亮点 Related products:
Malate Dehydrogenase antibodies; Malate Dehydrogenase ELISA Kits; Anti-Rabbit IgG secondary antibodies;
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预测分子量 36 kDa
翻译后修饰 ISGylated.
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.

检测图片 (3) Click the Picture to Zoom In

  • ARG55687 anti-MDH1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG55687 anti-MDH1 antibody.

  • ARG55687 anti-MDH1 antibody WB image

    Western blot: 35 µg of HL-60 cell lysate stained with ARG55687 anti-MDH1 antibody.

  • ARG55687 anti-MDH1 antibody FACS image

    Flow Cytometry: HL-60 cells stained with ARG55687 anti-MDH1 antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.