ARG55688
anti-MDH2 antibody
anti-MDH2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MDH2 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Bov, Rat, Mk, Pig, Yeast |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MDH2 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 86-115 (Center) of Human MDH2. |
| 偶联标记 | Un-conjugated |
| 別名 | MDH; MOR1; MGC:3559; Malate dehydrogenase, mitochondrial; EC 1.1.1.37; M-MDH |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P08249 Mouse Malate dehydrogenase, mitochondrial Swiss-port # P40926 Human Malate dehydrogenase, mitochondrial |
|---|---|
| 基因名称 | MDH2 |
| 全名 | malate dehydrogenase 2, NAD (mitochondrial) |
| 背景介绍 | Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] |
| 细胞定位 | Mitochondrion matrix. |
| 预测分子量 | 36 kDa |
| 翻译后修饰 | Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation by about 60%. |
检测图片 (3) Click the Picture to Zoom In
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ARG55688 anti-MDH2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma stained with ARG55688 anti-MDH2 antibody.
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ARG55688 anti-MDH2 antibody WB image
Western blot: 35 µg of HeLa cell lysate stained with ARG55688 anti-MDH2 antibody.
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ARG55688 anti-MDH2 antibody FACS image
Flow Cytometry: K562 cells stained with ARG55688 anti-MDH2 antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.
