ARG63943
anti-MDM2 antibody
anti-MDM2 antibody for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes MDM2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Cat, Rat, Cow, Dog, Pig |
| 应用 | WB |
| 特异性 | This antibody is expected to recognize isoform MDM2 (NP_002383.2) only. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MDM2 |
| 抗原物种 | Human |
| 抗原 | C-DELSGERQRKRHKSD |
| 偶联标记 | Un-conjugated |
| 別名 | EC 6.3.2.-; Double minute 2 protein; p53-binding protein Mdm2; hdm2; Oncoprotein Mdm2; HDMX; ACTFS; E3 ubiquitin-protein ligase Mdm2; Hdm2 |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 55 kDa |
| 翻译后修饰 | Phosphorylation on Ser-166 by SGK1 activates ubiquitination of p53/TP53. Phosphorylated at multiple sites near the RING domain by ATM upon DNA damage; this prevents oligomerization and E3 ligase processivity and impedes constitutive p53/TP53 degradation. Autoubiquitination leads to proteasomal degradation; resulting in p53/TP53 activation it may be regulated by SFN. Also ubiquitinated by TRIM13. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitination and degradation of p53/TP53. Deubiquitinated by USP7 leading to its stabilization. |
检测图片 (2) Click the Picture to Zoom In
-
ARG63943 anti-MDM2 antibody WB image
Western Blot: Human Liver lysate (35 µg protein in RIPA buffer) stained with ARG63943 anti-MDM2 (isoform) antibody at 0.3 µg/ml dilution.
-
ARG63943 anti-MDM2 antibody WB image
Western Blot: 1). Mock transfection; 2) MDM2 (RC219518) expressing plasmid transfected HEK293 cell lysate standed with ARG63943 anti-MDM2 (isoform) antibody
