ARG52333
anti-MEK1/2 phospho (Ser218 / 222) antibody
anti-MEK1/2 phospho (Ser218 / 222) antibody for Western blot and Human,Mouse
Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MEK1/2 phospho (Ser218 / 222) |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Bov, Rat, Chk, Dog, NHuPrm, Xenopus laevis, Zfsh |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MEK1/2 |
| 抗原物种 | Human |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | MEK 1; PRKMK1; MAPKK 1; EC 2.7.12.2; MEK1; MAPKK1; MKK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPK/ERK kinase 1; CFC3; ERK activator kinase 1 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~45k MEK 1/2 protein phosphorylated at Ser218/222 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P31938 Mouse Dual specificity mitogen-activated protein kinase kinase 1 Swiss-port # Q02750 Human Dual specificity mitogen-activated protein kinase kinase 1 |
|---|---|
| 基因名称 | MAP2K1/2 |
| 全名 | mitogen-activated protein kinase kinase 1 |
| 背景介绍 | MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. |
检测图片 (1) Click the Picture to Zoom In
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ARG52333 anti-MEK1/2 phospho (Ser218 / 222) antibody WB image
Western blot: NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum) stained with ARG52333 anti-MEK1/2 phospho (Ser218 / 222) antibody. Immunolabeling of an additional band at ~95kDa was also observed.
