ARG51512
anti-MEK2 phospho (Thr394) antibody
anti-MEK2 phospho (Thr394) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MEK2 phospho (Thr394) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MEK2 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from Human MEK-2. |
| 偶联标记 | Un-conjugated |
| 別名 | MEK 2; PRKMK2; MAPK/ERK kinase 2; EC 2.7.12.2; MAPKK2; MEK2; MAPKK 2; MAP kinase kinase 2; CFC4; MKK2; ERK activator kinase 2; Dual specificity mitogen-activated protein kinase kinase 2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MAP2K2 |
| 全名 | mitogen-activated protein kinase kinase 2 |
| 背景介绍 | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. |
| 生物功能 | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). [UniProt] |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 44 kDa |
| 翻译后修饰 | MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity). Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. |
检测图片 (3) Click the Picture to Zoom In
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ARG51512 anti-MEK2 phospho (Thr394) antibody WB image
Western blot: Extracts from HepG2 and HeLa cells untreated or treated with UV stained with ARG51512 anti-MEK2 phospho (Thr394) antibody.
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ARG51512 anti-MEK2 phospho (Thr394) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51512 anti-MEK2 phospho (Thr394) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51512 anti-MEK2 phospho (Thr394) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51512 anti-MEK2 phospho (Thr394) antibody.
