ARG51645
anti-MEK6 / MKK6 phospho (Ser207) antibody
anti-MEK6 / MKK6 phospho (Ser207) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MEK6 / MKK6 phospho (Ser207) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MEK6 / MKK6 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of serine 207 (V-D-S(p)-V-A) derived from Human MKK6. |
| 偶联标记 | Un-conjugated |
| 別名 | SAPK kinase 3; MEK 6; MAPKK 6; MAPK/ERK kinase 6; EC 2.7.12.2; PRKMK6; SAPKK3; MAPKK6; SAPKK-3; Stress-activated protein kinase kinase 3; MKK6; MAP kinase kinase 6; Dual specificity mitogen-activated protein kinase kinase 6; MEK6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P52564 Human Dual specificity mitogen-activated protein kinase kinase 6 Swiss-port # P70236 Mouse Dual specificity mitogen-activated protein kinase kinase 6 |
|---|---|
| 基因名称 | MAP2K6 |
| 全名 | mitogen-activated protein kinase kinase 6 |
| 背景介绍 | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively. |
| 生物功能 | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases. [UniProt] |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 37 kDa |
| 翻译后修饰 | Weakly autophosphorylated. Phosphorylated at Ser-207 and Thr-211 by the majority of M3Ks, such as MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2. Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. |
检测图片 (3) Click the Picture to Zoom In
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ARG51645 anti-MEK6 / MKK6 phospho (Ser207) antibody WB image
Western blot: Extracts from HL60 cells untreated or treated with PMA stained with ARG51645 anti-MEK6 / MKK6 phospho (Ser207) antibody.
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ARG51645 anti-MEK6 / MKK6 phospho (Ser207) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51645 anti-MEK6 / MKK6 phospho (Ser207) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51645 anti-MEK6 / MKK6 phospho (Ser207) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells showing cytoplasmic staining stained with ARG51645 anti-MEK6 / MKK6 phospho (Ser207) antibody.
