ARG56222
anti-MEK7 antibody
anti-MEK7 antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MEK7 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MEK7 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 363-396 of Human MEK7. |
| 偶联标记 | Un-conjugated |
| 別名 | c-Jun N-terminal kinase kinase 2; MEK; MEK 7; JNK-activating kinase 2; MAPKK 7; SAPK kinase 4; EC 2.7.12.2; PRKMK7; JNKK 2; JNK kinase 2; SAPKK-4; SAPKK4; MAPKK7; MAPK/ERK kinase 7; JNKK2; MAP kinase kinase 7; Stress-activated protein kinase kinase 4; MKK7; Dual specificity mitogen-activated protein kinase kinase 7 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O14733 Human Dual specificity mitogen-activated protein kinase kinase 7 |
|---|---|
| 基因名称 | MAP2K7 |
| 全名 | mitogen-activated protein kinase kinase 7 |
| 背景介绍 | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| 生物功能 | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. [UniProt] |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks). |
检测图片 (1) Click the Picture to Zoom In
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ARG56222 anti-MEK7 antibody WB image
Western blot: 20 µg of HeLa cell lysate stained with ARG56222 anti-MEK7 antibody at 1:2000 dilution.
