ARG54861
anti-MGMT antibody [888CT22.2.4]
anti-MGMT antibody [888CT22.2.4] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes MGMT |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 888CT22.2.4 |
| 同位型 | IgG1 |
| 靶点名称 | MGMT |
| 抗原 | Purified His-tagged MGMT protein. |
| 偶联标记 | Un-conjugated |
| 別名 | O-6-methylguanine-DNA-alkyltransferase; Methylated-DNA--protein-cysteine methyltransferase; MGMT; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | MCF7 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P16455 Human Methylated-DNA--protein-cysteine methyltransferase |
|---|---|
| 基因名称 | MGMT |
| 全名 | O-6-methylguanine-DNA methyltransferase |
| 背景介绍 | Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. MGMT is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015] |
| 生物功能 | MGMT involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProt] |
| 细胞定位 | Nucleus. |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 22 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG54861 anti-MGMT antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG54861 anti-MGMT antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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ARG54861 anti-MGMT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue stained with ARG54861 anti-MGMT antibody at 1:25 dilution.
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ARG54861 anti-MGMT antibody WB image
Western blot: 35 µg of MCF7 cell lysate stained with ARG54861 anti-MGMT antibody.
