ARG66313
anti-MGMT antibody [SQab1861]
anti-MGMT antibody [SQab1861] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1861] recognizes MGMT |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1861 |
| 同位型 | IgG |
| 靶点名称 | MGMT |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the N-terminus of Human MGMT. |
| 偶联标记 | Un-conjugated |
| 別名 | O-6-methylguanine-DNA-alkyltransferase; Methylated-DNA--protein-cysteine methyltransferase; MGMT; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P16455 Human Methylated-DNA--protein-cysteine methyltransferase |
|---|---|
| 基因名称 | MGMT |
| 全名 | O-6-methylguanine-DNA methyltransferase |
| 背景介绍 | Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. MGMT is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015] |
| 生物功能 | MGMT involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProt] |
| 产品亮点 | Related products: MGMT antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| 预测分子量 | 22 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG66313 anti-MGMT antibody [SQab1861] FACS image
Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:50 dilution (blue) in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control. .
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ARG66313 anti-MGMT antibody [SQab1861] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast tissue stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
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ARG66313 anti-MGMT antibody [SQab1861] WB image
Western blot: 20 µg of MCF7, Jurkat, HeLa and HT-29 cell lysates stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:5000 dilution.
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ARG66313 anti-MGMT antibody [SQab1861] IP image
Immunoprecipitation: 0.4 mg of HeLa whole cell lysate was immunoprecipitated (1:80 dilution) and stained with ARG66313 anti-MGMT antibody [SQab1861].
Lane 1: Immunoprecipitation in HeLa whole cell lysate
Lane 2: PBS instead of Primary Ab in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 µg (input)
